DNA-polycation nanospheres as non-viral gene delivery vehicles
Identifieur interne : 002765 ( Main/Exploration ); précédent : 002764; suivant : 002766DNA-polycation nanospheres as non-viral gene delivery vehicles
Auteurs : K. W Leong [États-Unis] ; H.-Q Mao [États-Unis] ; V. L Truong-Le [États-Unis] ; K. Roy [États-Unis] ; S. M Walsh [États-Unis] ; J. T August [États-Unis]Source :
- Journal of Controlled Release [ 0168-3659 ] ; 1998.
English descriptors
- Teeft :
- Aqueous solution, Bioactive, Bioactive materials, Brosis, Brosis transmembrane conductance regulator, Cftr, Cftr expression, Chemical scheme, Chitosan, Chloroquine, Chondroitin sulfate, Coacervation, Complex coacervation, Crosslinking, Cystic, Different batches, Different cell lines, Different formulations, Direct gene transfer, Drug delivery, Drug therapy, Electrophoretic mobility analysis, Electrostatic interaction, Form microspheres, Gelatin, Gelatin nanospheres, Gene, Gene delivery, Gene delivery system, Gene delivery vehicles, Gene therapeutics, Gene therapy, Gene transfer, Hela cells, Immune response, International symposium, Johns hopkins university, Lacz gene, Leong, Lipofectamine, Lipofectamine complexes, Loading level, Lower panel, Lysosomal compartments, Lysosomolytic agents, Molecular probes, Molecular weight, Multiple plasmids, Nanosphere, Nanosphere approach, Nanosphere formulations, Nanospheres, Nuclease degradation, Other bioactive agents, Phase separation, Phosphate buffer, Plain nanospheres, Plasmid, Release society, Room temperature, Serum exposure, Serum nuclease degradation, Smooth muscle cells, Sodium sulfate concentration, Transfection, Transferrin, Uncrosslinked nanospheres, Upper panel, Vaccine applications, Viral vectors.
Abstract
Abstract: Nanospheres synthesized by salt-induced complex coacervation of cDNA and polycations such as gelatin and chitosan were evaluated as gene delivery vehicles. DNA-nanospheres in the size range of 200–750 nm could transfect a variety of cell lines. Although the transfection efficiency of the nanospheres was typically lower than that of lipofectamine and calcium phosphate controls in cell culture, the β-gal expression in muscle of BALB/c mice was higher and more sustained than that achieved by naked DNA and lipofectamine complexes. This gene delivery system has several attractive features: (1) ligands can be conjugated to the nanosphere for targeting or stimulating receptor-mediated endocytosis; (2) lysosomolytic agents can be incorporated to reduce degradation of the DNA in the endosomal and lysosomal compartments; (3) other bioactive agents or multiple plasmids can be co-encapsulated; (4) bioavailability of the DNA can be improved because of protection from serum nuclease degradation by the polymeric matrix; (5) the nanosphere can be lyophilized for storage without loss of bioactivity.
Url:
DOI: 10.1016/S0168-3659(97)00252-6
Affiliations:
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T August</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology and Molecular Sciences, School of Medicine, Johns Hopkins University, Baltimore, MD 21205</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Controlled Release</title><title level="j" type="abbrev">COREL</title><idno type="ISSN">0168-3659</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">53</biblScope><biblScope unit="issue">1–3</biblScope><biblScope unit="page" from="183">183</biblScope><biblScope unit="page" to="193">193</biblScope></imprint><idno type="ISSN">0168-3659</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-3659</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aqueous solution</term><term>Bioactive</term><term>Bioactive materials</term><term>Brosis</term><term>Brosis transmembrane conductance regulator</term><term>Cftr</term><term>Cftr expression</term><term>Chemical scheme</term><term>Chitosan</term><term>Chloroquine</term><term>Chondroitin sulfate</term><term>Coacervation</term><term>Complex coacervation</term><term>Crosslinking</term><term>Cystic</term><term>Different batches</term><term>Different cell lines</term><term>Different formulations</term><term>Direct gene transfer</term><term>Drug delivery</term><term>Drug therapy</term><term>Electrophoretic mobility analysis</term><term>Electrostatic interaction</term><term>Form microspheres</term><term>Gelatin</term><term>Gelatin nanospheres</term><term>Gene</term><term>Gene delivery</term><term>Gene delivery system</term><term>Gene delivery vehicles</term><term>Gene therapeutics</term><term>Gene therapy</term><term>Gene transfer</term><term>Hela cells</term><term>Immune response</term><term>International symposium</term><term>Johns hopkins university</term><term>Lacz gene</term><term>Leong</term><term>Lipofectamine</term><term>Lipofectamine complexes</term><term>Loading level</term><term>Lower panel</term><term>Lysosomal compartments</term><term>Lysosomolytic agents</term><term>Molecular probes</term><term>Molecular weight</term><term>Multiple plasmids</term><term>Nanosphere</term><term>Nanosphere approach</term><term>Nanosphere formulations</term><term>Nanospheres</term><term>Nuclease degradation</term><term>Other bioactive agents</term><term>Phase separation</term><term>Phosphate buffer</term><term>Plain nanospheres</term><term>Plasmid</term><term>Release society</term><term>Room temperature</term><term>Serum exposure</term><term>Serum nuclease degradation</term><term>Smooth muscle cells</term><term>Sodium sulfate concentration</term><term>Transfection</term><term>Transferrin</term><term>Uncrosslinked nanospheres</term><term>Upper panel</term><term>Vaccine applications</term><term>Viral vectors</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Nanospheres synthesized by salt-induced complex coacervation of cDNA and polycations such as gelatin and chitosan were evaluated as gene delivery vehicles. DNA-nanospheres in the size range of 200–750 nm could transfect a variety of cell lines. Although the transfection efficiency of the nanospheres was typically lower than that of lipofectamine and calcium phosphate controls in cell culture, the β-gal expression in muscle of BALB/c mice was higher and more sustained than that achieved by naked DNA and lipofectamine complexes. This gene delivery system has several attractive features: (1) ligands can be conjugated to the nanosphere for targeting or stimulating receptor-mediated endocytosis; (2) lysosomolytic agents can be incorporated to reduce degradation of the DNA in the endosomal and lysosomal compartments; (3) other bioactive agents or multiple plasmids can be co-encapsulated; (4) bioavailability of the DNA can be improved because of protection from serum nuclease degradation by the polymeric matrix; (5) the nanosphere can be lyophilized for storage without loss of bioactivity.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Leong, K W" sort="Leong, K W" uniqKey="Leong K" first="K. W" last="Leong">K. W Leong</name></region><name sortKey="August, J T" sort="August, J T" uniqKey="August J" first="J. T" last="August">J. T August</name><name sortKey="Leong, K W" sort="Leong, K W" uniqKey="Leong K" first="K. W" last="Leong">K. W Leong</name><name sortKey="Mao, H Q" sort="Mao, H Q" uniqKey="Mao H" first="H.-Q" last="Mao">H.-Q Mao</name><name sortKey="Roy, K" sort="Roy, K" uniqKey="Roy K" first="K" last="Roy">K. Roy</name><name sortKey="Truong Le, V L" sort="Truong Le, V L" uniqKey="Truong Le V" first="V. L" last="Truong-Le">V. L Truong-Le</name><name sortKey="Walsh, S M" sort="Walsh, S M" uniqKey="Walsh S" first="S. M" last="Walsh">S. M Walsh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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